ULASIMANG BATO FREE

Metronidazole and DMSO served as positive and negative controls, respectively. The live trophozoites (unstained) were counted under a binocular microscope. The trophozoites were stained with Trypan blue and dispensed into chambers of a Neubauer hemocytometer. The amoeba and leaf extracts were incubated for 24, 48, and 72 hours. Anti-amoebic activity was determined in an in vitro assay using Entamoeba histolytica HK-9 strain against 10 dose levels (18-10,000 µg/mL). The tsaang gubat and ampalaya leaves were sourced from the University of the Philippines at Los Baños and processed into a lyophilized aqueous extract.

The aim of this research was to provide evidence to validate the use of tsaang gubat and ampalaya leaf extracts for dysentery by determining their anti-amoebic activity. Tsaang gubat and ampalaya have been used by traditional healers from different cultures to treat dysentery. Few drugs such as metronidazole are available to treat this disease unfortunately, it is associated with several serious side effects. Amoebiasis is a global health problem affecting poor regions in the world. there was no significant difference on the effect of Ulasimang Bato extract on Staphylococcus aureus.Ĭonclusion: The effectiveness of (Peperomia pellucida) Ulasimang Bato extract on the growth of Staphylococcusaureus as an antimicrobial agent was proven not effective by measuring its zone of inhibition it did not exert any significant effect against Penicillin and 10% Betadine Solution.Background. Since the computed mean of Ulasimang Bato is zero (0) therefore the null hypothesis is accepted. No further computation is needed to know the effectiveness of Ulasimang Bato extract on Staphylococcus aureus. The mean zone of inhibition of Betadine Solution is 3.2 centimeters and 0 centimeter for Ulasimang Bato. Result: The mean zone of ingibition of Penicillin is 8.8 centimeters showing greater effect in terms of its antimicrobial activity. It took 3 days to finish completely the experiment. There were five samples used for the experiment and all the materials used were properly sterilized through autoclaving. The funds gathered were used in buying all the necessary instruments for the experiment. The researchers gathered funds through personal sources.

The laboratory technicians were also informed in terms of details of the study for the accomplishment of the laboratory extraction specifically using the Soxhlet Apparatus. Procedures/Interventions: The researchers sent letters of request for using the Research Council Laboratory and Microbiology Laboratory and also in buying the standard organism.

ULASIMANG BATO FREE

Taking into considerations that subjects of this study are sterile and free from any contaminants. There is a sample of standard organism against five samples of sensitivity discs treated with Ulasimang Bato and being observed of its antimicrobial effectivity on the result of the growth of Staphylococcus aureus. Subjects: The subject of the study is a sample of standard organism of staphylococcus aureus. Setting: This study was conducted at the Research Council Laboratory and microbiology Laboratory of Cebu Doctors' College. Objective: The main thrust of this study is to determine the effect of Ulasimang Bato extract as an antimicrobial agent.ĭesign: This study utilized the experimental research design.

Ulasimang bato